Composite

Part:BBa_K3560005:Design

Designed by: ShiDi Xiao   Group: iGEM20_XHD-Wuhan-China   (2020-10-23)


PGroES-DrRBS-gabY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 399
    Illegal NgoMIV site found at 565
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Theoretically, after transforming gabY-pRADK to DR containing gcd-pRADK, the effective concentration of the whole enzyme formed by GDH and PQQ coenzymes will increase, and the catalytic efficiency will be higher.


Source

We know the sequence of the gabY gene, and synthesize the gabY gene fragment through a biological company. Then the gabY fragment was recombined with pRADK to form gabY-pRADK.

References